|
KMID : 0878220010130010051
|
|
Journal of Sasang Constitutional Medicine
2001 Volume.13 No. 1 p.51 ~ p.60
|
|
|
Effects of Taeumjowetang on Lipid Peroxidation by Free Radicals and Oxidative Damage of Hepatocytes by tert-Butyl Hydroperoxide
|
|
Park Seong-Sik
|
|
|
Abstract
|
|
|
Purpose: The present study was carried out to evaluate the antioxidant effects of Taeumjowetang in vitro.
Methods: In this study, antioxidant effects of TJT on lipid peroxidation were determined according to the method of TBA. (Abbreviation) TJT : Taeumjowetang, TBA : 2-thiobarbituric acid.
Results: 1) TJT inhibited markedly peroxidation of linoleic acid the autoxidation. 2) TJT inhibited lipid peroxidation induced by hydroxyl radical derived from H202-Fe2+ in rat liver homogenate. 3) TJT showed 66% scavenging effect on DPPH radical. 4) TJT exhibited a 25% inhibitory effect on superoxide generation from xanthine-xan thine oxidase system. 5) To investigate the antioxidative effects of TJT on the hepatocytes, cultured normal rat liver cells(Ac2F) were prepared and incubated with or without TJT. After 16∼16hr, cells placed in DMEM medium without serum, and then incubated with 1mM t-BHP for 2hr. Viable cells were detected by MTT assay. In this test, TJT protected the cell death induced by t-BHP and significantly increased cell viability in the normal rat liver cell. (Abbreviation) DPPH : α, α-diphenyl-β-picryl hydrazyl, DMEM : Dulbecco’s Modified Eagle Medium, t-BHP : tert-butyl hydroperoxide,
Conclusion: These results suggested that TJT might play a protective role in lipid peroxidation by free radicals.
|
|
|
KEYWORD
|
|
|
Taeumjowetang, Lipid Peroxidation, Damage of Hepatocytes, t-BHP
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|